| 质粒名称: | pEG302 5aa SunTag VP64 g4+g17 (FWA) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 119672 |
| 存储实验室: | Steven Jacobsen |
|
载体骨架基本信息 |
|
| 载体名称: | pEG302 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达，CRISPR，合成生物学 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS,5aa SunTag VP64 g4+g17 |
| 物种来源: | -- |
| 插入大小: | 15401 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pEG302 5aa SunTag VP64 g4+g17 (FWA) was a gift from Steven Jacobsen (Addgene plasmid # 119672 ; http://n2t.net/addgene:119672 ; RRID:Addgene_119672) |
| 参考文献部分: | Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. 10.1038/s41467-019-08736-7 PubMed 30760722 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pEG302 5aa SunTag VP64 g4+g17 (FWA) 25433 bp ds-DNA circular SYN 16-APR-2019
DEFINITION CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two
guide RNAs.
ACCESSION .
VERSION .
KEYWORDS pEG302 5aa SunTag VP64 g4+g17 (FWA)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 25433)
AUTHORS Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE
TITLE Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9
SunTag systems.
JOURNAL Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7.
PUBMED 30760722
REFERENCE 2 (bases 1 to 25433)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 16, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..25433
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 4616..4640
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 4659..4714
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 4728..8831
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 8835..8861
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 8880..8900
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8907..8927
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8991..9047
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9063..9119
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9135..9191
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9207..9263
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9279..9335
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9351..9407
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9423..9479
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9495..9551
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9567..9623
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9639..9695
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
terminator 9743..10450
/label=OCS terminator
/note="octopine synthase terminator"
promoter complement(10483..10501)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(10484..10501)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(10525..10542)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(10525..10541)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10534..10556)
/label=M13/pUC Forward
/note="In lacZ gene"
misc_feature 10744..10768
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 12069..12698
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 13127..14200
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 14266..14460
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(14696..14718)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 14804..14944
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 14859..14878
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(14959..14976)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(15130..15718)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(15210..15229)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(17142..17936)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 18361..18385
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 18475..18649
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(18690..19715)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(19782..20458)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
primer_bind complement(19825..19845)
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
misc_RNA complement(20543..20618)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_RNA complement(21097..21172)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
CDS complement(21923..21973)
/codon_start=1
/product="nuclear localization signal from the human T-cell
leukemia virus type 1 (HTLV-1) Rex protein"
/label=Rex NLS
/translation="PKTRRRPRRSQRKRPPT"
CDS complement(22001..22162)
/codon_start=1
/product="B1 domain of Streptococcal protein G "
/label=GB1
/note="effective as a solubilizing fusion partner (Cheng
and Patel, 2004)"
/translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
ATKTFTVTE"
CDS complement(22193..22213)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS complement(22238..22387)
/codon_start=1
/product="tetrameric repeat of the minimal activation
domain of herpes simplex virus VP16 (Beerli et al., 1998)"
/label=VP64
/translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
DLDML"
CDS complement(22427..23137)
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
VTAAGITHGMDELYK"
primer_bind complement(22467..22487)
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
primer_bind 23082..23110
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
CDS complement(23228..23254)
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
ORIGIN
1 agaagatgca gatgagtgga ttagaaggaa agaggctcag aatgtaagga aagcaccaac
61 cacaacaccg tcgggacaga cgagaaacat gggaagctcc accacaatct tgggttaagt
121 gcaactttga tggggcatgg ccaacagaag gattaaaatg tggcttaggg tgggtgcttc
181 gcgatcatac agggaaggtg ttatggttag gtgcaagagc tgtggtaaaa gtaagaagcg
241 tgctggaagt agaagtggag gctcttagat gggctgtgct gtcattatcc cgattcaatt
301 ataggaagat catttttgag gtggattctc agcaacttgt gtctttggtt acatgaaagt
361 tatgcttgtc aagtctcaat ccaattatcc aagacataaa gtatctactt agcaagtttg
421 aggattttat gcttgtgcat acaagccgag aaggaaatgg agtggcagat agaatagcta
481 aggaatctct ttcttttgag aattatgatc taaagttgta ttctattgta ccaatttggg
541 ttaaaagctc tgttgagcta gactcatatg catccctagg aaacaagttg taatgagttg
601 ctggcctctc ttgggattgt tcatcaaagt agctgtccct acacaccaca acagaatggt
661 gttgtggaga gaaagcacag acacatcctt gagatggcaa gggcacttaa gtttcagagt
721 ggtgtaccta ccaggttttg gggagactgt gtcaagactg ttgtatacct gatcaataga
781 ctccctactc caatcttaca aggcaaatgt ccatatgaac tactctatca gaaacatgcc
841 aagcttgatc acttgagagt gtttgggtgt cttgcttttg cagcaacact gcccaaaggt
901 gacaaacttg cacctagagc caagcgaacc atcttcattg gttattctga gactcaaaag
961 ggttacaggt tgtatgactt ggataataag gtgatcatag tgagcaggga tgtagttttc
1021 agagagtttc agttcccttt caaagagtcc atctcccatg aacctgatat gttcactcga
1081 gcaggttcac ttagctctga agatacagct ttgcagctag tgtataatga tatttttcct
1141 tccagctttc atactgatga tgatgatact ccagttctta ttcctgacat tgtagaggcc
1201 aatgaggata tcactttggg gactcattct acccaaaatc atatcacttc agctgatgct
1261 gcaccttcag gccacctgca tgctcctgca gagtcagttg agtcagctga gcctgaccta
1321 gcaaatgctg aatttgagca ccatactgct gtagctgatc cctctctagt tccagcaaac
1381 ctgcacacta gacctaaacg caatgcaggt cctcccatct ggctcaagga ctttgtgaca
1441 ctgaacaaag gctctaggga tgttccatac cctatagcca actatgtttc ctatgatcac
1501 ttgcctgttc actatcaagc ttatttgagt gctttctcca cttatactga acctaaatcc
1561 ttcaaagaag cagctcaaga tgagaaatgg atggaggcca tgtcccttga gatacaggct
1621 cttgaggata ataacacctg ggagattgtc cctttacccc ctggtaaaca gcctataggg
1681 tccaaatggg tgtacaaaat taaatacaaa gctaatggtg aagttgacag gtttaaggca
1741 aggctagtgg ccaagggata cactcagcaa gaaggccttg actaccatga aactttttct
1801 ccagtggcca aaatggtcac tgtaaaagct gtcatatctg ttgctgcttc caagggctgg
1861 ttccttttcc agatggatgt caacaatgct tttctacaag gtgacctcat ggaagaagtt
1921 tatatgtctc tgcctcaggg ttttcacagc caaggggaga ccaaagtgtg caggctgttg
1981 aatccctcta tggtttaaag caagcatcaa ggcagtggaa catcaagctt accactgtcc
2041 ttatgcaggc tggttttatg caaagtgctt atgatcactc cttgtttacc aaaagagagg
2101 ggactgacct tgtcataatc ctgatttatg tggatgatct gttaataaca ggcagcagca
2161 atgttttgat ttcagaagca aaggcaaccc tgcatcagca tttcaagatg aaagatttgg
2221 gagaactaaa atacttctta ggcattgagg tgctgagatc agaaaaggga atcctactga
2281 accagaggaa gtatgcactg gaactgatat caggtgttgg tctgggaggc tgcagaccag
2341 tgtcaacacc aatggaacag aaccaaaggc tgactactgt agaatatgac aagcacttag
2401 gaaagactga tgatgcagag ttagaggatg tgggttccta tcagagactg gtgggaaagc
2461 ttctctactt gacaatcaca aggccagata tcagctttgc agtgcaggtg ctgagtcaat
2521 tcatgcagca acccaaacag tcacacctgg aagcagcatt gagggttgtg aagtacataa
2581 aaggttctcc aggtgttagg aacgcggccg catgcatatg agtctagctc aacagagctt
2641 ttaacccaaa ttggtacaat agaatacaac tttagatcat aattctcaaa agaaagagat
2701 tccttagcta ttctatctgc cactccattt ccttctcggc ttgtatgcac aagcataaaa
2761 tcctcaaact tgctaagtag atactttatg tcttggataa ttggattgag acttgacaag
2821 cataactttc atgtaaccaa agacacaagt tgctgagaat ccacctcaaa aatgatcttc
2881 ctataattga atcgggataa tgacagcaca gcccatctaa gagcctccac ttctacttcc
2941 agcacgcttc ttacttttac cacagctctt gcacctaacc ataacacctt ccctgtatga
3001 tcgcgaagca cccaccctaa gccacatttt aatccttctg ttggccatgc cccatcaaag
3061 ttgcacttaa cccaagattg tggtggagct tcccatgttt ctcgtctgtc ccgacggtgt
3121 tgtggttggt gctttcctta cattctgagc ctctttcctt ctaatccact catctgcatc
3181 ttcttgtgtc cttactaata cctcattggt tccaaattcc ctccctttaa gcaccagctc
3241 gtttctgttc ttccacagcc tcccaagtat ccaagggact aaagcctcca cattcttcag
3301 atcaggatat tcttgtttaa gatgttgaac tctatggagg tttgtatgaa ctgatgatct
3361 aggaccggat aagttccctt cttcatagcg aacttattca aagaatgttt tgtgtatcat
3421 tcttgttaca ttgttattaa tgaaaaaata ttattggtca ttggactgaa cacgagtgtt
3481 aaatatggac caggccccaa ataagatcca ttgatatatg aattaaataa caagaataaa
3541 tcgagtcacc aaaccacttg ccttttttaa cgagacttgt tcaccaactt gatacaaaag
3601 tcattatcct atgcaaatca ataatcatac aaaaatatcc aataacacta aaaaattaaa
3661 agaaatggat aatttcacaa tatgttatac gataaagaag ttacttttcc aagaaattca
3721 ctgattttat aagcccactt gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa
3781 ataaagcacg aagaattcta gaaaatacga aatacgcttc aatgcagtgg gacccacggt
3841 tcaattattg ccaattttca gctccaccgt atatttaaaa aataaaacga taatgctaaa
3901 aaaatataaa tcgtaacgat cgttaaatct caacggctgg atcttatgac gaccgttaga
3961 aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca
4021 cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat
4081 tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc
4141 tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc
4201 ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc aatttctcaa
4261 aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct
4321 tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg
4381 gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg ttagatatca
4441 tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt
4501 tgtcgaataa ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt
4561 tgtgcgatcg aatttgtcga ttaatctgag tttttctgat taacagggat catcaacaag
4621 tttgtacaaa aaagcaggct ctttaaagta tttttacaac aattaccaac aacaacaaac
4681 aacaaacaac attacaatta ctatttacaa ttacaaaaaa agttaacatg gacaagaagt
4741 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
4801 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
4861 agaacctgat cggcgccctg ctgttcgaca gcggagaaac agccgaggcc acccggctga
4921 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
4981 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
5041 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
5101 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
5161 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
5221 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
5281 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
5341 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
5401 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg attgccctga
5461 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
5521 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
5581 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
5641 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
5701 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
5761 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacatcgatg
5821 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
5881 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
5941 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
6001 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
6061 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
6121 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
6181 gcgccagcgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
6241 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtac aacgagctga
6301 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
6361 aaaaagccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
6421 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
6481 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
6541 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
6601 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
6661 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
6721 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
6781 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
6841 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
6901 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
6961 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
7021 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
7081 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
7141 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
7201 accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc gtgcctcaga
7261 gctttctgaa ggacgactcc atcgataaca aagtgctgac tcggagcgac aagaaccggg
7321 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcgcc
7381 agctgctgaa tgccaagctg attacccaga ggaagttcga caatctgacc aaggccgaga
7441 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
7501 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
7561 agaacgacaa actgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
7621 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
7681 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
7741 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
7801 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
7861 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
7921 caaacggcga aacaggcgag atcgtgtggg ataagggccg ggactttgcc accgtgcgga
7981 aagtgctgtc tatgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
8041 tcagcaaaga gtctatcctg cccaagagga acagcgacaa gctgatcgcc agaaagaagg
8101 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
8161 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
8221 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
8281 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
8341 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
8401 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
8461 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaaacact
8521 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
8581 ctaatctgga caaggtgctg agcgcctaca acaagcacag agacaagcct atcagagagc
8641 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
8701 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
8761 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
8821 tgggaggcga cgcctatccc tatgacgtgc ccgattatgc cagcctgggc agcggctccc
8881 ccaagaaaaa acgcaaggtg gaagatccta agaaaaagcg gaaagtggac ggcattggta
8941 gtgggagcaa cggcagcagc ggatccaacg gtccgactga cgccgcggaa gaagaacttt
9001 tgagcaagaa ttatcatctt gagaacgaag tggctcgtct taagaaaggt tctggcagtg
9061 gagaagaact gctttcaaag aattaccacc tggaaaatga ggtagctaga ctgaaaaagg
9121 ggagcggaag tggggaggag ttgctgagca aaaattatca tttggagaac gaagtagcac
9181 gactaaagaa agggtccgga tcgggtgagg agttactctc gaaaaattat catctcgaaa
9241 acgaagtggc tcggctaaaa aagggcagtg gttctggaga agagctatta tctaaaaact
9301 accacctcga aaatgaggtg gcacgcttaa aaaagggaag tggcagtggt gaagagctac
9361 tatccaagaa ttatcatctt gagaacgagg tagcgcgttt gaagaagggt tccggctcag
9421 gagaggaact gctctcgaag aactatcatc ttgaaaatga ggtcgctcga ttaaaaaagg
9481 gatcgggcag tggtgaggaa ctactttcaa agaattacca cctcgaaaac gaagtagctc
9541 gattaaagaa aggttcaggg tcgggtgaag aattactgag taaaaattat catctggaaa
9601 atgaggtagc gagactaaaa aaggggagtg gttctggcga ggaattgcta tcgaaaaatt
9661 atcatcttga gaacgaagtt gctaggctca aaaagggctc aggctcaggc taacccgggg
9721 gcgcgccatg ataactagag tcctgcttta atgagatatg cgagacgcct atgatcgcat
9781 gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga
9841 tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt
9901 atgaataata ttctccgttc aatttactga ttgtacccta ctacttatat gtacaatatt
9961 aaaatgaaaa caatatattg tgctgaatag gtttatagcg acatctatga tagagcgcca
10021 caataacaaa caattgcgtt ttattattac aaatccaatt ttaaaaaaag cggcagaacc
10081 ggtcaaacct aaaagactga ttacataaat cttattcaaa tttcaaaagt gccccagggg
10141 ctagtatcta cgacacaccg agcggcgaac taataacgtt cactgaaggg aactccggtt
10201 ccccgccggc gcgcatgggt gagattcctt gaagttgagt attggccgtc cgctctaccg
10261 aaagttacgg gcaccattca acccggtcca gcacggcggc cgggtaaccg acttgctgcc
10321 ccgagaatta tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac
10381 cttgacagtg acgacaaatc gttgggcggg tccagggcga attttgcgac aacatgtcga
10441 ggctcagcag gaccggcatg caagctagct tactagtgat attctatagt gtcacctaaa
10501 tctctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
10561 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
10621 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
10681 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
10741 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
10801 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
10861 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
10921 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
10981 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
11041 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
11101 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
11161 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
11221 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
11281 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
11341 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
11401 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
11461 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
11521 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
11581 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
11641 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
11701 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
11761 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
11821 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
11881 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
11941 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
12001 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
12061 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
12121 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
12181 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
12241 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
12301 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
12361 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
12421 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
12481 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
12541 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
12601 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
12661 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
12721 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
12781 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
12841 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
12901 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
12961 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
13021 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
13081 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
13141 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
13201 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
13261 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
13321 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
13381 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
13441 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
13501 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
13561 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
13621 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
13681 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
13741 gcgtacaaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
13801 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgaact
13861 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
13921 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
13981 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
14041 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
14101 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
14161 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
14221 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
14281 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
14341 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
14401 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
14461 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
14521 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
14581 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
14641 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
14701 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
14761 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
14821 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
14881 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
14941 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
15001 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
15061 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
15121 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
15181 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
15241 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
15301 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
15361 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
15421 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
15481 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
15541 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
15601 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
15661 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
15721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
15781 gctgatgaat cccctaatga tttttatcaa aatcattaag ttaaggtaga tacacatctt
15841 gtcatatgat caaatggttt cgccaaaaat caataatcag acaacaaaat gtgcgaactc
15901 gatattttac acgactctct ttaccaattc tgccccgaat tacacttaaa acgactcaac
15961 agcttaacgt tggcttgcca cgccttactt gactgtaaaa ctctcactct taccgaactt
16021 ggccgtaacc tgccaaccaa agcgagaaca aaacataaca tcaaacgaat cgaccgattg
16081 ttaggtaatc gtcacctcca caaagagcga ctcgctgtat accgttggca tctagcttta
16141 tctgttcggg caatacgatg cccattgtac ttgttgactg gtctgatatc cgtgagcaaa
16201 aacggcttat ggtattgcga gcttcagtcg cactacacgg tcgttctgtt actctttatg
16261 agaaagcgtt cccgctttca gagcaatgtt caaagaaagc tcatgaccaa tttctagccg
16321 accttgcgag cattctaccg agtaacacca caccgctcat tgtcagtgat gctggcttta
16381 aagtgccatg gtataaatcc gttgagaagc tgggttggta ctggttaagt cgagtaagag
16441 gaaaagtaca atatgcagac ctaggagcgg aaaactggaa acctatcagc aacttacatg
16501 atatgtcatc tagtcactca aagactttag gctataagag gctgactaaa agcaatccaa
16561 tctcatgcca aattctattg tataaatctc gctctaaagg ccgaaaaaat cagcgctcga
16621 cacggactca ttgtcaccac ccgtcaccta aaatctactc agcgtcggca aaggagccat
16681 ggattctagc aactaactta cctgttgaaa ttcgaacacc caaacaactt gttaatatct
16741 attcgaagcg aatgcagatt gaagaaacct tccgagactt gaaaagtcct gcctacggac
16801 taggcctacg ccatagccga acgagcagct cagagcgttt tgatatcatg ctgctaatcg
16861 ccctgatgct tcaactaaca tgttggcttg cgggcgttca tgctcagaaa caaggttggg
16921 acaagcactt ccaggctaac acagtcagaa atcgaaacgt actctcaaca gttcgcttag
16981 gcatggaagt tttgcggcat tctggctaca caataacaag ggaagactta ctcgtggctg
17041 caaccctact agctcaaaat ttattcacac atggttacgc tttggggaaa ttatgagggg
17101 atctctcagc gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt
17161 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
17221 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
17281 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
17341 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
17401 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
17461 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
17521 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
17581 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
17641 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
17701 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
17761 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
17821 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
17881 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
17941 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
18001 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
18061 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc
18121 ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
18181 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
18241 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
18301 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
18361 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
18421 gacgttttta atgtactgaa ttaacgccga attaattcga gctctaattc gggggatctg
18481 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
18541 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg
18601 aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt
18661 cgatcgacag atcccggtcg gcatctactc tatttctttg ccctcggacg agtgctgggg
18721 cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt
18781 ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat
18841 cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg
18901 tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg atcctgcaag ctccggatgc
18961 ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag
19021 atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct
19081 gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc
19141 cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac
19201 gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg
19261 catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac
19321 ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tagcctccgc gaccggttgt
19381 agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgaacggcgg
19441 gagatgcaat aggtcaggct ctcgctaaac tccccaatgt caagcacttc cggaatcggg
19501 agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
19561 ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
19621 tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc
19681 tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc attgcccccc ggatctgcga
19741 aagctcgaga gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa
19801 tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca
19861 tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg
19921 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
19981 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
20041 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
20101 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
20161 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttcac atcaatccac
20221 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
20281 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
20341 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
20401 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
20461 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
20521 ctccaccatg gtaccaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga
20581 ctagccttat tttaacttgc tatttctagc tctaaaactc catggatggc ctagttttca
20641 atcactactt cgactctagc tgtatataaa ctcagcttcg ttttcttatc taagcgatgt
20701 gggacttttg aagattgttt tcaacttaaa tgggcctata taagaaatac tattgttctt
20761 tcccatataa atgggcctgc ttctcttctt tcagattccc aggggccttt tgaagattat
20821 cttcatatct taagaatgaa gatgttttat tcaatcaaat tcttgaaggt tcgatgccta
20881 atcattctaa tcctgggaca aactatgaaa caagatacaa aaactccgaa tggaaagtta
20941 aaaagaagaa aacgaaagct acggttcaag aaaatgtaag ctgataaaca aaaaaaaact
21001 gtatgaacga agaagaagaa aaaaagctaa gaagaaatga tgtattgtgc ggaaggcaag
21061 tcgagtttcc gttgttcaac gaaagcttca aaaaaagcac cgactcggtg ccactttttc
21121 aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa acaagcccat
21181 acatctttcc gtcaatcact acttcgactc tagctgtata taaactcagc ttcgttttct
21241 tatctaagcg atgtgggact tttgaagatt gttttcaact taaatgggcc tatataagaa
21301 atactattgt tctttcccat ataaatgggc ctgcttctct tctttcagat tcccaggggc
21361 cttttgaaga ttatcttcat atcttaagaa tgaagatgtt ttattcaatc aaattcttga
21421 aggttcgatg cctaatcatt ctaatcctgg gacaaactat gaaacaagat acaaaaactc
21481 cgaatggaaa gttaaaaaga agaaaacgaa agctacggtt caagaaaatg taagctgata
21541 aacaaaaaaa aactgtatga acgaagaaga agaaaaaaag ctaagaagaa atgatgtatt
21601 gtgcggaagg caagtcgagt ttccgttgtt caacgaaagc ttcgtaacat agatgacacc
21661 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
21721 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
21781 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
21841 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc ggggaaattc
21901 gagctcaaac ttacggccat ggcgtaggcg gccttttccg ttgtgatctt cgcggtctcc
21961 tccttgtttt tggactagta ccaccaccgc taccaccacc ttcggttacc gtgaaggttt
22021 tggtagcgtc gtcgtaggtc cattcaccgt caacaccgtt gtcgttagcg tactgtttga
22081 aaactttttc cgcggtagca gcgtcaacag cttcggtggt ggtttcacct ttcagggttt
22141 taccgttcag gataagcttg tactcttcgg tccgagatcc tcctcccgta cggacctttc
22201 tcttcttctt tggagcccct ccgccaccgc cactacctag catatctaga tcaaagtcat
22261 ctagggcatc ggagcccaac atatctaaat cgaagtcgtc caatgcatct gagcctaaca
22321 tgtctaggtc gaagtcgtct aacgcgtctg aaccaagcat atctaagtca aaatcgtcta
22381 aagcatcccc tccgccaccg cccgtacgac cggtccgacc tccacctttg tagagctcat
22441 ccatgccatg tgtaatccca gcagcagtta caaactcaag aaggaccatg tggtcacgct
22501 tttcgttggg atctttcgaa aggacagatt gtgtcgacag gtaatggttg tctggtaaaa
22561 ggacagggcc atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacggaac
22621 catcttcaac gttgtggcga attttgaagt tagctttgat tccattcttt tgtttgtctg
22681 ccgtgatgta tacattgtgt gagttaaagt tgtactcgag tttgtgtcca agaatgtttc
22741 catcttcttt aaaatcaata ccctttaact cgatacgatt aacaagggta tcaccttcaa
22801 acttgacttc agcacgcgtc ttgtaggtcc cgtcatcttt gaaagatata gtgcgttcct
22861 gtacataacc ttcgggcatg gcactcttga aaaagtcatg ccgtttcatg tgatccggat
22921 aacgggaaaa gcattgaaca ccataggtca gagtagtgac aagtgttggc cacggaacag
22981 gtagttttcc agtagtgcaa ataaatttaa gggtgagttt tccgtttgta gcatcacctt
23041 caccctctcc acggacagaa aatttgtgcc cattaacatc accatctaat tcaacaagaa
23101 ttgggacaac tccagtgaaa agttcttctc ctttgctacc gcttccacct ccacctggat
23161 ccaagcttcc gccgcctcct gaaccaccac cgccactacc tcctccccca gaacctccgc
23221 ctccaccagc gtaatctgga acatcgtatg ggtagctgct cacggtcacc agggtgccct
23281 ggccccagta gtcgaacagg ccggtcacgc agtagtacag ggcggtgtcg tcgctgcgca
23341 ccttgctcat ctgcaggtac acggtgttct tgccgttgtc cttgctgatg atgaagcggt
23401 ccttcagggc gctgttgtag tcggtgatgc cgtcgcccca gatcacgccg atccactcca
23461 ggccgcggcc gggggcctgg cgcacccagt tcacgccgta gtcggtcagg ctgaagccgc
23521 tcacggcgca gctcagcttc aggctgccgc cgggctgcac caggccgccg ccgctctcca
23581 gcagcttcac ctcgctgccg ccgccgctgc tgccgccgcc gccgctgccg ccgccgccgc
23641 tgccgccgcc gccgcgcttc agctccacct tggtgccctg gccgaacacc cagtggttgc
23701 tgtaccacag ggcgcagaag taggtggcga agtcctcggg ctgcaggctg ctgatggtca
23761 gggtggcctt gtcgccgatc aggctgccgc tgaagcggct gggcacgccg ggggcgcggt
23821 tgttggtgcc gccgatcagg cccttgaaca gcttgccggg cttctcctgc acccagctgg
23881 cgtagttgct ggtggtcacg gcgccggtgc tgctgcggca ggtgatggtc acgcggtcgc
23941 ccacgctggc gctcaggctg ctggggctct gggtcatcac gatgtcgggg cccatcagcg
24001 ctaaacccta ggtgttaatc agaaaaactc agattaatcg acaaattcga tcgcacaaac
24061 tagaaactaa caccagatct agatagaaat cacaaatcga agagtaatta ttcgacaaaa
24121 ctcaaattat ttgaacaaat cggatgatat ctatgaaacc ctaatcgaga attaagatga
24181 tatctaacga tcaaacccag aaaatcgtct tcgatctaag attaacagaa tctaaaccaa
24241 agaacatata cgaaattggg atcgaacgaa aacaaaatcg aagattttga gagaataagg
24301 aacacagaaa tttaccttga tcacggtaga gagaattgag agaaagtttt taagattttg
24361 agaaattgaa atctgaattg tgaagaagaa gagctctttg ggtattgttt tatagaagaa
24421 gaagaagaaa agacgaggac gactaggtca cgagaaagct aaggcggtga agcaatagct
24481 aataataaaa tgacacgtgt attgagcgtt gtttacacgc aaagttgttt ttggctaatt
24541 gccttatttt taggttgagg aaaagtattt gtgctttgag ttgataaaca cgactcgtgt
24601 gtgccggctg caaccacttt gacgccgttt attactgact cgtcgacaac cacaatttct
24661 aacggtcgtc ataagatcca gccgttgaga tttaacgatc gttacgattt atattttttt
24721 agcattatcg ttttattttt taaatatacg gtggagctga aaattggcaa taattgaacc
24781 gtgggtccca ctgcattgaa gcgtatttcg tattttctag aattcttcgt gctttatttc
24841 ttttcctttt tgtttttttt tgccatttat ctaatgcaag tgggcttata aaatcagtga
24901 atttcttgga aaagtaactt ctttatcgta taacatattg tgaaattatc catttctttt
24961 aattttttag tgttattgga tatttttgta tgattattga tttgcatagg ataatgactt
25021 ttgtatcaag ttggtgaaca agtctcgtta aaaaaggcaa gtggtttggt gactcgattt
25081 attcttgtta tttaattcat atatcaatgg atcttatttg gggcctggtc catatttaac
25141 actcgtgttc agtccaatga ccaataatat tttttcatta ataacaatgt aacaagaatg
25201 atacacaaaa cattctttga ataagttcgc tatgaagaag ggaacttatc cggtcctaga
25261 tcatcagttc atacaaacct ccatagagtt caacatctta aacaagaata tcctgatctg
25321 aagaatgtgg aggctttagt cccttggata cttgggaggc tgtggaagaa cagaaacgag
25381 ctggtgctta aagggaggga atttggaacc aatgaggtat tagtaaggac aca
//
